Coding

Part:BBa_K2027053:Design

Designed by: Gordon Sun   Group: iGEM16_Stanford-Brown   (2016-10-24)


H. Brasiliensis SRPP cDNA (codon Optimized)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 19


Design Notes

This sequence has been acquired from H. brasiliensis and codon optimized for transfection into E. coli. Mutation A486T was induced to remove a restriction site.

The stop codon has been removed from this sequence to allow for C terminus concatenation with tags.

A FLAG-Lumio tag can be appended to the sequence to allow for visualization and/or purification.

Source

Sequence was sourced from Oh SK, Kang H, Shin DH, et al. Isolation, characterization, and functional analysis of a novel cDNA clone encoding a small rubber particle protein from Hevea brasiliensis. J Biol Chem. 1999;274(24):17132-8. (http://www.ncbi.nlm.nih.gov/nuccore/AF051317)

References